Review





Similar Products

chx  (MedChemExpress)
97
MedChemExpress chx
Chx, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chx/product/MedChemExpress
Average 97 stars, based on 1 article reviews
chx - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
cycloheximide hy n0901  (MedChemExpress)
94
MedChemExpress cycloheximide hy n0901
Pathogenic variant damaged the stability of RPE65 possibly through <t>the</t> <t>ubiquitination–proteasome</t> pathway. ( A ) When HEK293T cells were transfected with WT variant plasmids for 24 hours and treated with 100-µM <t>CHX,</t> RPE65 expression was detected. ( B ) RPE65 stability was detected after cells were treated with 30-µM MG-132. ( C ) Twenty-four hours after transfection, WT mutant proteins were enriched by immunoprecipitation, and their ubiquitination levels were detected.
Cycloheximide Hy N0901, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cycloheximide hy n0901/product/MedChemExpress
Average 94 stars, based on 1 article reviews
cycloheximide hy n0901 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
cycloheximide chx  (MedChemExpress)
97
MedChemExpress cycloheximide chx
Pathogenic variant damaged the stability of RPE65 possibly through <t>the</t> <t>ubiquitination–proteasome</t> pathway. ( A ) When HEK293T cells were transfected with WT variant plasmids for 24 hours and treated with 100-µM <t>CHX,</t> RPE65 expression was detected. ( B ) RPE65 stability was detected after cells were treated with 30-µM MG-132. ( C ) Twenty-four hours after transfection, WT mutant proteins were enriched by immunoprecipitation, and their ubiquitination levels were detected.
Cycloheximide Chx, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cycloheximide chx/product/MedChemExpress
Average 97 stars, based on 1 article reviews
cycloheximide chx - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
chx hy 12320  (MedChemExpress)
97
MedChemExpress chx hy 12320
Pathogenic variant damaged the stability of RPE65 possibly through <t>the</t> <t>ubiquitination–proteasome</t> pathway. ( A ) When HEK293T cells were transfected with WT variant plasmids for 24 hours and treated with 100-µM <t>CHX,</t> RPE65 expression was detected. ( B ) RPE65 stability was detected after cells were treated with 30-µM MG-132. ( C ) Twenty-four hours after transfection, WT mutant proteins were enriched by immunoprecipitation, and their ubiquitination levels were detected.
Chx Hy 12320, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chx hy 12320/product/MedChemExpress
Average 97 stars, based on 1 article reviews
chx hy 12320 - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier

Image Search Results


Pathogenic variant damaged the stability of RPE65 possibly through the ubiquitination–proteasome pathway. ( A ) When HEK293T cells were transfected with WT variant plasmids for 24 hours and treated with 100-µM CHX, RPE65 expression was detected. ( B ) RPE65 stability was detected after cells were treated with 30-µM MG-132. ( C ) Twenty-four hours after transfection, WT mutant proteins were enriched by immunoprecipitation, and their ubiquitination levels were detected.

Journal: Translational Vision Science & Technology

Article Title: Pathogenicity and Functional Analysis of Multi-Variant Allele of RPE 65 Causing Retinitis Pigmentosa

doi: 10.1167/tvst.15.2.1

Figure Lengend Snippet: Pathogenic variant damaged the stability of RPE65 possibly through the ubiquitination–proteasome pathway. ( A ) When HEK293T cells were transfected with WT variant plasmids for 24 hours and treated with 100-µM CHX, RPE65 expression was detected. ( B ) RPE65 stability was detected after cells were treated with 30-µM MG-132. ( C ) Twenty-four hours after transfection, WT mutant proteins were enriched by immunoprecipitation, and their ubiquitination levels were detected.

Article Snippet: After 24 hours of transfection, HEK293T cells were treated with 100-μM cycloheximide (CHX, HY-N0901; MedChemExpress, Monmouth Junction, NJ) or CHX mixed with MG-132 proteasome inhibitor (HY-12320; MedChemExpress).

Techniques: Variant Assay, Ubiquitin Proteomics, Transfection, Expressing, Mutagenesis, Immunoprecipitation